细胞凋亡
化学
细胞生物学
活性氧
线粒体
细胞内
膜电位
胞浆
生物学中的钙
细胞
线粒体内膜
钙
细胞生长
分子生物学
生物化学
生物
有机化学
酶
作者
De-biao Chi,Lin Li,Lisha Sun,Wenfeng Ma
摘要
OBJECTIVE To investigate the cell biological mechanism of koumine-induced apoptosis of human colonic adenocarcinoma cells. METHODS The effects of LoVo cell koumine on the membrane potential, mitochondrial membrane potential, concentration of cytosolic free calcium, reactive oxygen species (ROS) and gap junctional intercellular communication was observed by laser scanning confocal microscopy. RESULTS AND CONCLUSION Koumine lowered the membrane potential and mitochondrial membrane potential of LoVo cells and decreased the concentration of free cytosolic calcium, which was increased to a level higher than the basal one after addition of EDTA. Koumine also increased the reactive oxygen species and enhanced the gap junctional intercellular communication of LoVo cells. These findings may explain the possible mechanisms of koumine-induced LoVo cell apoptosis.
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