Study on extraction and degradation effect of enzyme which can degrade Aflatoxin B_1

Objective:The aim of this text was mainly to extract the main ingredients from the Stenotrophomonas sp.which can degrade aflatoxin B1.Method:Using ammonium sulfate precipitation method for enzymatic extraction,and optimize the conditions of enzyme degraded reaction.Result:Use 65% ammonium sulfate precipitation after 24h fermentation,the degradation ratio of AFB1 reached 80.25%,which was obviously higher than others and the enzyme had the greatest activity.Conclusion:The degraded micro-organisms fermentation at 40℃ for 24h,its protein content was 532.9μg /mL after 65% ammonium sulfate precipitation.The degradation ratio of AFB1 reached 81.23% after 72h reaction.This enzyme had absorption peaks in the 240～267nm through a full-wavelength scan.