Determination of the content of soluble sugar in sweet corn with optimized anthrone colorimetric method.

Taking sweet corn as test material,through the study of anthrone concentration,the temperature and time of color reaction of anthrone-furfuralx,the reaction conditions of the anthrone colorimetric method to determine the soluble sugar content of sweet corn were studied.The results showed that,the optimum conditions of anthrone colorimetric method were as follows: the optimum concentration of anthrone was 15 mg/mL,temperature of color reaction was 80 ℃,heating time was 15 min.Under these conditions,there was a good linear relationship between concentration of the glucose standard and absorbance(y =15.954 x +0.097 6,R2=0.999 2).With this optimized method,the determination results of the soluble sugar in sweet corn had good repeatability(RSD 0.47%) and accuracy(average recovery rate 101.92%).