Dual-responsive nisin loaded chondroitin sulfate nanogel for treatment of bacterial infection in soft tissues

Chondroitin sulfate-Nisin nanogels (CS-N NGs) were prepared by electrostatic interaction for nisin delivery as an antibacterial agent in the treatment of bacterial infections caused by some clinical strains of methicillin-resistant and methicillin-sensitive Staphylococcus aureus (S. aureus). The physical properties of CS-N NGs were evaluated using Fourier-transform infrared spectroscopy, dynamic light scattering, and field emission scanning electron microscopy. The average diameter of obtained CS-N NGs was about 65 nm and the stability of nanogels was assessed by zeta potential measurement. Enzyme and pH-responsibility of CS-N NGs due to the presence of susceptible bonds in chondroitin sulfate resulted in effective and controlled release of nisin in the simulated infectious medium. Also, the ability of prepared CS-N NGs for eradicating clinical methicillin resistance S. aureus strain was confirmed by Broth Microdilution Method (BMD) and the cytotoxicity analysis was carried out on Human Dermal Fibroblast (HDF) cells by MTT assay method. Based on the results, this versatile drug carrier could efficiently deliver the cationic antimicrobial peptides as a natural antibiotic for growth inhibition of methicillin-resistant S. aureus strains and further destroying the bacteria in the treatment of subcutaneous infections caused by methicillin-resistant S. aureus strains.