糖基化
N-连接糖基化
聚糖
糖蛋白
化学
计算生物学
功能(生物学)
生物化学
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2271: 1-21
被引量:2
标识
DOI:10.1007/978-1-0716-1241-5_1
摘要
Glycosylation is a common posttranslational modification of therapeutic proteins. The glycosylation pattern is dependent on many parameters such as the host cell line or the culture conditions. N- and O-linked glycans usually play a great role on the stability, safety, and efficacy of the drug. For this reason, glycosylation is considered as a critical quality attribute of therapeutic glycoproteins, and a thorough characterization should be performed, as well as a systematic control for each batch produced. This chapter gives a short presentation of the structure of glycans commonly found on recombinant therapeutic proteins, and their role on the properties of the drug, in terms of stability, pharmacokinetics, safety, and efficacy. Lastly, the use of mass spectrometry for the analysis of glycoproteins is briefly described.
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