[Metabolic regulation in constructing microbial cell factories].

The regulation of the expression of genes involved in metabolic pathways, termed as metabolic regulation, is vital to construct efficient microbial cell factories. With the continuous breakthroughs in synthetic biology, the mining and artificial design of high-quality regulatory elements have substantially improved our ability to modify and regulate cellular metabolic networks and its activities. The research on metabolic regulation has also evolved from the static regulation of single genes to the intelligent and precise dynamic regulation at the systems level. This review briefly summarizes the advances of metabolic regulation technologies in the past 30 years.代谢调控是构建微生物细胞工厂的重要技术手段。随着合成生物学技术的不断突破，挖掘和人工设计的高质量调控元件大幅度提升了对细胞代谢网络的改造能力；代谢调控研究也已从单基因的静态调控发展到系统水平上的智能精确动态调控。文中简要综述了近30年来代谢途径表达调控技术在代谢工程领域的研究进展。.