生物
Skp1型
F盒蛋白
基因
亚细胞定位
遗传学
功能(生物学)
蛋白质结构域
细胞生物学
泛素连接酶
非生物胁迫
基因家族
蛋白质家族
基因表达
泛素
作者
Jiaojiao Zhang,Xinman Wang,Xiaonan Dong,Fei Wang,Lei Cao,Shuangtao Li,Ziji Liu,Xichun Zhang,Yang‐Dong Guo,Bing Zhao,Na Zhang
出处
期刊:Plant Science
[Elsevier]
日期:2022-11-01
卷期号:324: 111454-111454
标识
DOI:10.1016/j.plantsci.2022.111454
摘要
Tubby-like protein (TLP) plays an important role in plant growth and development. In this investigation, the characteristics of 11 members in the SlTLP family were studied. SlTLP genes were classified into two subgroups, and the members containing the F-box domain were renamed SlTLFPs. Subcellular localization indicated that most of the SlTLPs were localized in the nucleus. Expression pattern analysis revealed that eight genes (SlTLFP1, 3, 5, 7-10, and SlTLP11) showed differential expression across various tissues, while SlTLFP2, 4, and 6 were widely expressed in all the organs tested. Most SlTLP genes were induced by biotic and abiotic stress treatments such as Botrytis cinerea, temperature, MeJA, and ABA. TLP proteins in tomato have no transcriptional activation activity, and most members with an F-box domain could interact with SUPPRESSOR OF KINETOCHORE PROTEIN 1 (SlSkp1) or Cullin1 (Cul1) or both. Experiments on CRISPR edited SlTLFP8 showed that the N-terminal F-box domain was necessary for its function such as DNA ploidy and stomata size regulation. Our findings suggested that the F-box domain interacts with Skp1 and Cul1 to form the SCF complex, suggesting that SlTLFPs, at least SlTLFP8, function mainly through the F-box domain as an E3 ligase.
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