Bioengineered nanomaterials for dynamic diagnostics in vivo

. Firstly, an input energy source with high tissue penetration is needed, such as near-infrared (NIR) light, X-rays, magnetic field and ultrasound. Secondly, a nanomaterial class that is responsive to such an energy source to provide a readable output signal is chosen. Thirdly, bioengineered nanoprobes are designed to exhibit spatial, temporal or spatiotemporal changes in the output signal. Finally, different methods are used to analyse the output signal of nanoprobes, such as detecting changes in optical, radiation, magnetic and ultrasound signals. This review also discusses the obstacles and potential solutions for advancing these bioengineered nanomaterials toward clinical translational applications.