亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Orderly Regulation of Macrophages and Fibroblasts by Axl in Bleomycin‐Induced Pulmonary Fibrosis in Mice

博莱霉素 肺纤维化 纤维化 炎症 病理 癌症研究 医学 特发性肺纤维化 免疫学 内科学 化疗
作者
Xinyu Zhao,Yupeng Li,Shengnan Yang,Yicong Chen,Kaiwei Wu,Jing Geng,Peipei Liu,Zai Wang,Huaping Dai,Chen Wang
出处
期刊:Journal of Cellular and Molecular Medicine [Wiley]
卷期号:29 (1) 被引量:2
标识
DOI:10.1111/jcmm.70321
摘要

ABSTRACT Pulmonary fibrosis is a pathological manifestation that occurs upon lung injury and subsequence aberrant repair with poor prognosis. However, current treatment is limited and does not distinguish different disease stages. Here, we aimed to study the differential functions of Axl, a receptor tyrosine kinase expressing on both macrophages and fibroblasts, in the whole course of pulmonary fibrosis. We used mice with Axl total knockout, conditionally knockout in macrophages or fibroblasts, or treating with Axl inhibitors in inflammation or fibrosis stages to examine the effect of temporary dysfunction of Axl on bleomycin (BLM)‐induced pulmonary fibrosis. Primary bone marrow–derived monocytes and primary fibroblasts from mice were used for cell‐type–specific studies. Lung tissue and plasma samples were collected from idiopathic pulmonary fibrosis (IPF) patients and healthy controls to assess the Axl levels. We found that Axl inhibited the M1 polarisation of macrophages; inhibition of Axl during acute phase exacerbated inflammatory response and subsequent pulmonary fibrosis. On the other hand, Axl promoted the proliferation and invasion of the fibroblasts, partially by accelerating the focal adhesion turnover; inhibiting Axl during the fibrotic phase significantly alleviated pulmonary fibrosis. Consistently, phosphorylated Axl levels increased in fibrotic foci in the lung sample of IPF patients. In contrast, the soluble Axl (sAxl) level decreased in their plasma as compared to healthy controls. These results indicate that Axl may sequentially and differentially regulate macrophages and fibroblasts in acute and fibrosis phases, implying the necessity of a stage‐specific treatment for pulmonary fibrosis. In addition, the activated Axl on fibroblasts may be reflected by the lowered plasma sAxl level, which may act as a biomarker for IPF. Trial Registration: ClinicalTrials.gov identifier: NCT03730337
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
真的不会完成签到,获得积分10
7秒前
香蕉觅云应助聪明凌丝采纳,获得10
8秒前
10秒前
yyh发布了新的文献求助30
16秒前
20秒前
hairgod发布了新的文献求助10
26秒前
31秒前
小马甲应助CryBill采纳,获得10
35秒前
cyclone发布了新的文献求助10
36秒前
36秒前
41秒前
44秒前
47秒前
47秒前
xia发布了新的文献求助10
50秒前
彭于晏应助年轻纸飞机采纳,获得10
51秒前
CryBill发布了新的文献求助10
52秒前
帅气灭绝发布了新的文献求助10
54秒前
葱葱完成签到,获得积分10
56秒前
58秒前
277发布了新的文献求助10
1分钟前
1分钟前
顾矜应助dogontree采纳,获得10
1分钟前
1分钟前
Zzk完成签到,获得积分10
1分钟前
1分钟前
babe完成签到 ,获得积分10
1分钟前
1分钟前
Allen0520完成签到,获得积分10
1分钟前
dogontree发布了新的文献求助10
1分钟前
生动梦松应助vvvvkeke采纳,获得30
1分钟前
1分钟前
1分钟前
田様应助邵邵采纳,获得10
1分钟前
NXNJ发布了新的文献求助10
1分钟前
wongtx完成签到,获得积分10
1分钟前
11完成签到,获得积分10
1分钟前
1分钟前
清新的宛丝完成签到,获得积分10
1分钟前
科研通AI5应助11采纳,获得10
1分钟前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Nuclear Fuel Behaviour under RIA Conditions 500
Sociologies et cosmopolitisme méthodologique 400
Why America Can't Retrench (And How it Might) 400
Another look at Archaeopteryx as the oldest bird 390
Parenchymal volume and functional recovery after clamped partial nephrectomy: potential discrepancies 300
Optimization and Learning via Stochastic Gradient Search 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 物理化学 基因 催化作用 遗传学 冶金 电极 光电子学
热门帖子
关注 科研通微信公众号,转发送积分 4681514
求助须知:如何正确求助?哪些是违规求助? 4057361
关于积分的说明 12544940
捐赠科研通 3752527
什么是DOI,文献DOI怎么找? 2072450
邀请新用户注册赠送积分活动 1101534
科研通“疑难数据库(出版商)”最低求助积分说明 980855