生物膜
化学
活性氧
葡萄糖氧化酶
微生物学
伤口愈合
胞外聚合物
NADPH氧化酶
免疫系统
细菌
生物化学
生物
免疫学
酶
遗传学
作者
Guangye Ge,Lijuan Wu,Fenglan Zhang,Tianyi Wang,Lei Han,Xiaoying Kong,Jinsheng Shi
标识
DOI:10.1016/j.ijbiomac.2022.11.265
摘要
In recent years, photodynamic therapy (PDT) or chemodynamic therapy (CDT) based on the antimicrobial property or anti-biofilm property of reactive oxygen species (ROS) have been widely recognized for their low susceptibility to microbial resistance. However, due to the complication of the three-dimensional structure of the biofilm at the wound site and the high quenching rate of common ROS, the treatment with traditional ROS could not achieve satisfactory wound healing effects. Here, Na2S2O8@ZIF-67/GOx nanoparticles (NZG NPs) were prepared as a new high-toxic ROS nanogenerator for application of biofilm-infecting wound healing with the assistance of glucose oxidase (GOx) for amplified CDT and immune activation. When the NZG NPs entered the biofilm, Co-based metal organic frame (ZIF-67) ruptured in the acidic microenvironment, which induced the release of GOx and the production of gluconic acid and H2O2, further promoting the decrease of pH of the biofilm microenvironment and in turn accelerating the cleavage of ZIF-67 and the release of Na2S2O8. Then, S2O82− could gradually transformed into high-toxic sulfate radical (SO4−), part of which further produced OH in situ with H2O, thereby inhibiting the proliferation of bacteria and biofilms. Interestingly, these two types of ROS not only caused direct damage to the biofilm, but also activated the immune system of the wound site as well as the body more effectively, which also played an indirect role in promoting biofilm destruction and wound healing. In vitro and in vivo results showed that, as a new high-toxic ROS nanogenerator, the NZG NPs supply amplified chemodynamic therapy and immune activation to destroy biofilms, but also achieve effective wound healing without causing bacterial tolerance, which provides a new strategy for the development of biofilm-infecting wound healing.
科研通智能强力驱动
Strongly Powered by AbleSci AI