PHÁT HIỆN GENE BẢN THỂ OXA-51-LIKE CỦA Acinetobacter baumannii BẰNG PHƯƠNG PHÁP REAL-TIME PCR

DETECTION OF INTRINSIC OXA-51-LIKE GENE IN Acinetobacter baumannii BY REAL-TIME PCR METHODAcinetobacter baumannii is one of leading causes of nosocomial infection. The bacteria possess instrinsic OXA-51-like gene. Moreover, with this gene the bacteria could resist to many antibiotics.The study aims to develop a real-time PCR protocol to detect intrinsic OXA-51-like gene in A. baumannii. The real-time PCR has been optimized with DNA-binding dye (EvaGreen) for amplifying OXA-51-like gene specific to A. baumannii and 16S rRNA gene specific to Acinetobacter spp. in one reaction. The protocol after setup was applied with several A. baumannii DNA samples. The result shows that the real-time PCR protocol can correctly detect intrinsic OXA-51-like gene in A. baumannii with the sensitivity of 32 copies/reaction and high specificity. The ratio of A. baumannii detection reached 100% for DNA samples which A. baumannii had been detected. The real-time PCR protocol using EvaGreen to detect OXA-51-like gene in A. baumannii has been developed successfully. The protocol can be used to rapidly detect A. baumannii based on this gene.