酮
代谢工程
产量(工程)
吹覆盆子
发酵
合成生物学
化学
无细胞蛋白质合成
无细胞系统
酮体
生物化学
酶
有机化学
食品科学
蛋白质生物合成
生物
计算生物学
材料科学
新陈代谢
冶金
作者
Simon Moore,Tomasso Tosi,David Bell,Yonek B Hleba,Karen Polizzi,Paul S. Freemont
摘要
Abstract Cell-free synthetic biochemistry provides a green solution to replace traditional petroleum or agricultural based methods for production of fine chemicals. 4-(4-hydroxyphenyl)-butan-2-one, also known as raspberry ketone, is the major fragrance component of raspberry fruit and is utilised as a natural additive in the food and sports industry. Current industrial processing standards involve chemical extraction with a yield of 1-4 mg per kilo of fruit. As such its market price can fluctuate up to $20,000 per kg. Metabolic engineering approaches to synthesise this molecule by microbial fermentation have only resulted in low yields of up to 5 mg L −1 . In contrast, cell-free synthetic biochemistry offers an intriguing compromise to the engineering constraints provided by the living cell. Using purified enzymes or a two-step semisynthetic route, an optimised pathway was formed for raspberry ketone synthesis leading up to 100% yield conversion. The semi-synthetic route is potentially scalable and cost-efficient for industrial synthesis of raspberry ketone.
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