A new fiber optic sensor for simultaneous measurement of temperature and saccharinity with multiplexed polymer-coated fiber Bragg gratings is proposed and demonstrated. It has been found that the polyimide-coated fibre Bragg gratings respond to variations of both temperature and saccharinity while the acrylate-coated fiber Bragg grating is only sensitive to the environmental temperature. The experimental results showed that temperature and saccharinity sensitivities of the multiplexed FBG sensor are 0.0102 nm/°C and 0.0012 nm/°Bx, respectively.