小眼畸形相关转录因子
川芎嗪
黑色素瘤
MAPK/ERK通路
黑色素
血管生成
污渍
化学
肿瘤坏死因子α
癌症研究
炎症
角质形成细胞
药理学
细胞培养
生物
医学
信号转导
免疫学
生物化学
酪氨酸酶
病理
替代医学
遗传学
基因
酶
作者
Gyoseon Goya M. Yeom,Seona Min,Sun Yeou Kim
标识
DOI:10.1016/j.intimp.2013.11.028
摘要
2,3,5,6-Tetramethylpyrazine (TMP) is known as a composition of Ephedra sinica and it has been used in the treatment of several disorders such as asthma, heart failure, rhinitis, and urinary incontinence. It has been reported that TMP inhibits melanoma metastasis and suppression angiogenesis by VEGF. The inhibitory activity of melanogenic proteins by TMP was confirmed in UVA-induced melanoma/keratinocyte co-culture system in this paper. The melanin content, cell viability and cytokines release such as TNFα, IL-1β, IL-8 and GM-CSF were measured by ELISA assay. In addition, TRP1, MITF and MAPK signaling protein expression were also evaluated by Western blotting analysis. Decreasing melanogenic factors (TRP1, MITF, and MAPK) and factors (TNFα, IL-1β, IL-8, and GM-CSF) improving skin cancer and inflammation were identified. It suggests that TMP can serve as a potent candidate for regulation of melanogenesis.
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