성장생물학 : Human Growth Hormone ( hGH ) 과 Porcine GH ( pGH ) 가 쥐와 돼지의 지방합성에 미치는 영향

The current study was undertaken to investigate he effects of human growth hormone(hGH) and porcine GH(pGH) on lipogenesis of adipose tissues from the rats and growing pigs. For the measurement of lipogenesis, epididymal fat tissue was taken from the rats after being killed and for the same purpose the backfat tissue was biopsyed from the pigs. Lipogenesis was determined by the amount of glucose converted to the total lipid. Initially, we investigated the effects of incubation time and glucose concentration on lipogenesis of the epididymal fat tissue of the rats for establishing the optimum condition. The results showed that lipogenesis was linearly increased as the incubation time increased (40, 80 and 120 min) and the total amount of lipogenesis was the same between 5mM and 20mM glucose in the KRB buffer. In in vitro study, adipose tissues were exposed to the varying concentrations of hGH during 48h culture; before the tissues were incubated in the KRB buffer for lipogenesis measurement. Human growth hormone added into the adipose tissue culture did not affect the lipogenesis of epididymal fat tissue of the rats. In in vivo study where the rats were injected daily with 200㎍ of hGH for 10 days, hGH added into the epididymal fat tissue culture did not affect the lipogenesis either. However, pGH as well as hGH contained in the culture media decreased the lipogenesis of the adipose tissue from growing pigs in dose-dependent manner. These results indicated that hGH decreased lipogenesis in the pigs, but not in the rats.