This chapter focuses on the process of one-dimensional (1D) nanomaterials assembly assisted by shear force of microflow, that is, the microflow-assisted or microfluidic 1D nanomaterial manipulation and assembly. It specifically focuses on the recent advances that are able to realize precise control of density, width, and location of assembled nanowires (NWs) array through combination of both fluidic shear force within the well-designed microfluidic channels and the mechanism of hydrodynamic focusing (HF). The chapter reviews a recent advance in this area that is able to precisely position a single NW onto predetermined locations with designated orientation using the mechanism of HF, which acts as microfluidic "hydro-tweezers" to ensure parallel NWs assembly and scalable integration of NW-based devices. It provides a brief introduction on present NWs assembly routes and focuses on one promising microfluidic assembly technique that utilizes the mechanism of HF.