Immunogenicity of EHF vaccine after injection or intragastric administration in mice

Objective To evaluate the observe immunogenicity of EHF administrated through two different administration routes for finding suitability dose and route. Methods Balb/c mice were immunized with EHF defunct vaccine at 0, 5, and 10 d by intragastric and hype injection, respectively. Five days after the last immunization, serum and intestine wash were detected with IFA. Results After immunization the injected groups had the special immune response to EHF vaccine. The special antibody was detected in serum and small intestine, but no special antibody was detected in intragastric group have. EHF vaccine of 1.4 TCID_ 50 could induce positive IgG and IgA. Conclusion After immunization with EHF vaccine of 1.4 TCID_ 50 , the injected group had the special immune response and secreted anti-EHF antibody, including IgG and IgA.