Determination of Fangchinoline and Tetrandrine in Compound Kendir Leaves Tablets by HPLC

Objective To determine the content of Fangchinoline and Tetrandrine in Compound Kendir Leaves tablets by HPLC,And to control the content of the Stephaniae Tetrandrae Radix.Methods The Hypersil ODS 2 was used as the column(5 μm,4.6 mm×250 mm).The mobile phase was acetonitrile-methanol-water-acetic acid(49∶23∶28∶1)(lauryl sodium sulfate 0.41 g/100 ml).The flow rate was 1.0 ml/min.The detection wavelength was at 280nm.The column temperature was 35℃.The sample volume was 10 μl.Methyl alcohol was used as the solvent with ultrasound 30 min,Fangchinoline and Tetrandrine in Compound Kendir Leaves tablets was extracted.Results Fangchinoline showed a good linear relationship in the range of 19.04～304.64 μg/ml.the regression equation was y=7 758.4x-3.471 9(r=1.000 0,n=5),The average recovery rate was 99.3%(n=6).Tetrandrine showed a good linear relationship in the range of 16.16～258.56 μg/ml,the regression equation was y=6 194.4x-0.238 4(r=1.000 0,n=5),the average recovery rate was 99.4%(n=6).Conclusion The method is accurate,and can be used for the content determination of Fangchinoline and Tetrandrine in Compound Kendir Leaves tablets.