Glucose Consumption Assay for the Evaluation of Ethanol-producing Capability of Microzyme

A new method for the evaluation of ethanol-producing capability of microzyme had been developed.In the experiments,while microzyme cells were cultured in cell culture mediums under aerobic conditions,ethanol-producing microzyme strains YE-1,YE-2 and YE-3 were cultured for alcohol fermentation in liquid culture mediums under anaerobic conditions.The amount of glucose consumption in alcohol fermentation liquid mediums was detected by spectrophotometric assay.Then alcohol concentration was calculated according to the amount of glucose consumption for alcohol fermentation.The alcohol concentration in these three alcohol fermentation alcohol mediums were 4.03 mg/mL,3.50 mg/mL and 3.77 mg/mL respectively,and then ethanol-producing capability of each strain was revealed.