The Clinical Study of the Methylation in Gene P16 in Early Diagnosis in Stomach Cancer

Objective:To detect methylation in p16 gene in whole blood and tissue of stomach cancer, and to explore its significance for early diagnosis in stomach cancer.Method:44 tissue samples and their corresponding whole blood samples from patients with stomach cancer, 20 tissue samples with gastric ulcer were collected for methylation measurement by PCR technique.Result:Methylated p16 gene was found in 38.6%(17/44)of stomach cancer and in (88.2%)(15/17) whole blood samples, no methylated p16 gene was found in gastric ulcer. Methylated p16 gene promoter in whole blood strongly correlated with tissue of stomach cancer.Conclusion:The measurement of methylated p16 gene should be helpful to early diagnosis in stomach cancer.