Establishment of A Muti-Drug Resistance Cell Line Derived from Human T Lymphocyte Leukemia Cells and Its Biological Features

Objective:To establish a multi-drug resistance(MDR) cell line Jurkat/Doxorubicin(DOX) derived from human T lymphocyte leukemia cell line Jurkat,and to compare the biological characteristics between Jurkat/DOX and Jurkat cell line.Methods:Jurkat cells were induced with long-term,singular,and dose-increasing DOX addition to MDR cell line Jurkat/DOX.The biological characteristics and the spectrum of MDR of Jurkat/DOX were evaluated by MTT assay.The intracellular accumulation of DOX was detected by flow cytometer and laser scanning confocal microscope.The expression of mdr1 was analyzed by RT-PCR.Western blot was also performed to detect the expression of P-gp.Results:MDR cell line Jurkat/DOX was established by long-term,intermittent,singular and dose-increasing DOX induction.The growth of Jurkat/DOX was slower than that in Jurkat cell line.Jurkat/DOX cells were resistant not only to the original DOX,but also to many other anticancer drugs such as CTX,VCR,and DNR.Intracellular accumulation of DOX was significantly less than that in parent cells.The expression of mdr1 gene and P-gp protein were up-regulated in Jurkat/DOX cell line.Conclusion:The MDR cell line Jurkat/DOX was successfully established,it has the property of resistant to many anticancer drugs,and the resistant maybe closely related to the over-expression of P-gp.