Construction of tissue-engineered heart valve by seeding human umbilical vein endothelial cells on decellular porcine aortic valve

Objective:To develop the tissue-engineered heart valve(TEHV) by seeding human umbilical vein endothelial cells(HUVECs) on decellular porcine aortic valve and to investigate its anti-coagulation function. Methods:The porcine aortic valve was decellularized using Trypsin.EDTA.Triton X-100.RNase and DNase treatment. HUVECs were cultured and seeded on decelluar valve to create TEHV in vitro. Histology and growth condition of cells were observed. Contents of tissue plas-minogen activator (t-PA) and type 1 plasminogen activator inhibitor (PAI-1) of endothelial cells were determined with the methods of colorsubstrate. Results:In vitro TEHV by seeding HUVECs on decellular porcine aortic valve was created successfully. Endothelial cells formed a continuous monolayer on the surface of valve and the cells can secrete t-PA and PAI-1. ConcuIsion:TEHV can be created using HUVECs as seed cells seeded on decellular porcine aortic valve, and the endothelial cells can secrete t-PA and PAI-1.