Effect of Rumen Fluid-buffer Ratio on Gas Production and pH in Short-term Rumen Simulation Technique

The short-term rumen simulation technique inoculated with different rumen fluid level was used to study effect of rumen fluid(rumen micro-organism) level on gas production and pH in the artificial rumen.Ten treatments,i.e.,artificial saliva: rumen fluid by 1∶1,2∶1,3∶1,4∶1,5∶1,6∶1,7∶1,8∶1,9∶1,10∶1 were used.Each treatment had 2 replicates and 2 blanks.Gas production was recorded after 2,4,8,12,24 and 48 h of fermentation,respectively,and pH was measured at the end.Results showed that gas production in the first 12 h had significant correlations with the rumen fluid level,especially that within the beginning 4 h,while gas production over 12 h and pH are irrelative to rumen fluid level.