Objective Study in diagnosis of neonatal septicemia,combined detection of clinical value of procalcitonin and high sensitivity C reactive protein.Methods From 2013 October-2014 year in June in our hospital 105 cases of neonatal sepsis group,including 47 cases of neonatal septicemia,control group was 58 healthy newborns,determination of content of reduced level of procalcitonin and high sensitivity C reactive protein in serum of two neonates.Results In neonatal sepsis group,high sensitive C reactive protein and procalcitoninserum level content respectively(26.9 + 17.8) mg /L and(13.5 + 10.1) ng / mL,the detection result is significantly higher than the control group levels.with statistical significance(P0.05).PCT CRP for the early diagnosis of neonatal sepsis,test,negative predictive value,no significant difference(P0.05);PCT sensitivity,specificity,positive predictive value and accuracy were higher than that of HS- CRP(P0.05).Conclusion In the clinical early diagnosis of neonatal sepsis,detection of neonatal drop levels of procalcitonin and high sensitivity C reactive protein,reference combination can be more effective on evaluation.