Studies on the cell suspension culture of Eucommia ulmoides Oliv. and its metabolite-aucubin

The production of aucubin in cell suspension culture of Eucommia ulmoides Oliv. was reported. Some physical and chemical factors affected the cell growth and the production of aucubin were investigated. The results showed that the concentration of aucubin was the highest on 18th day. The optimal conditions such as MS medium and the proper pH was 5.8 could produce 40.56 mg/L aucubin. 2.0 mg/L 2,4-D, NAA and 6-BA below 1.0 mg/L could promote the production of aucubin,but KT restrained the production of aucubin.