Antioxidant substances were extracted from mulberry vinegar with methanol ,ethanol, acetone and ethyl acetate respevtively. Their antioxidant activities were determined. The results show that the extracts of mulberry vinegar have more significant effects on DPPH radical scavenging than TBHQ. The different extracts have different antioxidant capacities, that is methanol extractantethanol extractant acetone extractantethyl acetate extractantTBHQ.