3-(N-甲基胺基)甲基兒茶酚及3-胺基甲基兒茶酚與偏鄰苯二酚雙加氧酶的反應

The results of cell culture experiments using semi-continuous fermentation system indicated that the benzoate is the limited species of the growth media. Another limited of growth factor is the oxygen supply. When the benzoate concentration was 9 g/L and the air supply was 5 L/min with the agitation speed of 600 rpm, a high yield (15g/L) of Pseudomonas cells can be obtained. The Ki’s of 3-chlorocatechol, 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol against metapyrocatechase were 1.86μM, 65.98μM and 85.48μM, respectively. The Km’s of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol are 53.94μM and 70.93μM, respectively. These results indicated that the inhibition ability of the substituted catechol is government by the size of substituent in the 3-position of catechol. The inactivation constant of 3-aminomethyl catechol and 3-(N-methylamino)methyl catechol was also examined. The Kinact of these two inactivators are 0.24 min-1 and 0.20 min-1, respectively.