食管癌
DNA甲基化
异位表达
癌症研究
体外
癌症
流式细胞术
甲基化
抑制器
生物
分子生物学
医学
细胞培养
DNA
基因表达
内科学
基因
生物化学
遗传学
作者
Hui Shi,Shengluan Zhou,Junhua Liu,Zhu Jun,Jianhua Xue,Luo Gu,Yijiang Chen
标识
DOI:10.1016/j.prp.2016.02.019
摘要
Increasing studies demonstrate that reduced expression of miR-34a is involved in the initiation and progression of cancers, and it has been characterized as a tumor suppressor in various types of cancers. In present study, we investigated the expression and role of miR-34a in esophageal cancer. qRT-PCR assays were performed to analyze the expression of miR-34a in human esophageal cancer tissues and adjacent esophageal tissues. CCK8 assay, flow cytometry analysis and in vitro migration assays were performed to analyze the role of miR-34a in human esophageal cancer cell. MSP assay was performed to analyze the DNA methylation of the miR-34a promoter. The expression of miR-34a was down-regulated in human esophageal cancer tissues. miR-34a ectopic expression affected esophageal cancer cells survival, proliferation and capabilities of migration in vitro. p53 status was not correlated with miR-34a. Subsequently, aberrant DNA methylation of the miR-34a promoter was found in human esophageal cancer, and 5-AZA-dC inhibited DNA methylation of the miR-34a promoter. our data showed that miR-34a acted as a tumor suppressor in human esophageal cancer.
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