生物
蛋白酶体
细胞生物学
转基因水稻
突变体
水稻
蛋白质降解
生物化学
作者
Xiao-Hong Hu,Jing Fan,Jin-Long Wu,Shen S,He J,Jiawen Liu,Hanyu Wang,Yong Zhu,Guo-Bang Li,Ji-Qun Zhao,Jie Xu,Diyan Li,Mei Pu,Zhi-Xue Zhao,Shi-Xin Zhou,Ji-Wei Zhang,Yan-Yan Huang,Yan Li,Yanli Lu,Fu Huang,Wenming Wang
标识
DOI:10.1101/2021.03.03.433750
摘要
ABSTRACT Crops with broad-spectrum resistance (BSR) to diseases are highly desirable in agricultural production. Identification of BSR loci and dissection of the underlying mechanisms are fundamental for crop resistance breeding. Here, we describe the identification and characterization of a rice UMP1 allele, which confers race-nonspecific BSR against blast pathogen Magnaporthe oryzae. OsUMP1 encodes a proteasome maturation factor that contributes to 26S proteasome abundance and activity in rice. Modulation of OsUMP1 expression leads to proteome changes, particularly affects the amounts and activities of H2O2-degrading enzymes. Consequently, H2O2 accumulation and disease resistance are enhanced in OsUMP1-overexpressing rice but reduced in loss-of-function mutants. Elevation of OsUMP1 expression also promotes rice resistance to foliar pathogens Rhizoctonia solani and Xanthomonas oryzae pv. oryzae and a floral pathogen Ustilaginoidea virens without observable yield penalty. These results indicate a BSR pathway linking the proteasome machinery and H2O2 homeostasis, and provide a candidate gene for balancing BSR and yield traits in rice breeding. One Sentence Summary A natural allele of rice UMP1 promotes resistance to multiple pathogens by boosting H2O2 accumulation.
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