模拟体液
磷灰石
生物相容性
傅里叶变换红外光谱
核化学
材料科学
结晶
MTT法
纳米复合材料
硅酸钙
扫描电子显微镜
化学工程
生物陶瓷
生物活性玻璃
化学
矿物学
有机化学
纳米技术
冶金
复合材料
细胞生长
生物化学
工程类
作者
Konstantina Kazeli,Ioannis Tsamesidis,Anna Theocharidou,Lamprini Malletzidou,Jonathan Rhoades,Georgia K. Pouroutzidou,Eleni Likotrafiti,K. Chrissafis,T. Lialiaris,Lambrini Papadopoulou,Eleana Kontonasaki,Evgenia Lymperaki
出处
期刊:Ceramics
[Multidisciplinary Digital Publishing Institute]
日期:2021-11-27
卷期号:4 (4): 628-651
被引量:7
标识
DOI:10.3390/ceramics4040045
摘要
Glass-ceramic nanopowder with a composition of 55SiO2-35CaO-10MgO (mol %) was synthesized by the sol–gel method and was heat treated at three temperatures (T1 = 835 °C, T2 = 1000 °C, T3 = 1100 °C) in order to obtain different materials (C1, C2, C3, respectively) varying in crystal structure. Bioactivity and oxidative stress were evaluated in simulated body fluid (SBF) for various time periods (up to 10 days). The structure of the synthesized materials and their apatite-forming ability were investigated by X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy and Energy Dispersive Spectroscopy (SEM/EDS). The antibacterial properties of the synthesized materials were evaluated against three Gram-positive and four Gram-negative bacterial strains and their biocompatibility was verified on a primary cell line of human gingival fibroblasts (HGFs) by the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay. The crystallization of the materials was increased by sintering temperature. Heat treatment did not inhibit the bioactive behavior of the materials as apatite formation started after 3 days in SBF. C2, C3 showed some indications of apatite forming even from the first day. Regarding cell viability, a variety of biological behaviors, concerning both dose and time points, was observed between the positive control and the tested materials by both the MTT assay and oxidative stress analysis. In conclusion, the nanobioceramic materials of this study possess a multitude of attractive physicochemical and biological properties that make them suitable candidates for bone regeneration applications, fillers in nanocomposite scaffolds, or as grafts in bone cavities and periodontal lesions.
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