Sensitive Determination of Deflazacort by Comparative Nano-Voltammetric and Ion Selective Potentiometric Analysis in Pharmaceuticals and Biologicals

The FDA approved Deflazacort (DZC) as a corticosteroid which is analogous of Prednisolone but with minimum side effects and more effective uses. A comparison between nano-voltammetric (Method A) and ion selective potentiometric (Method B) electro-analysis of DZC have been approached. In method A, reduction behavior of DZC was studied using square wave voltammetry (SWV) by both nano-gold electrode (NGE) and modified nano-screen printed electrode (NSPE) with Tetrakis; triphenylphosphine palladium (TK-NSPE) using Britton Robinson buffer at pH 4.0 were applied. To enhance the sensitivity of nano-voltammetric technique all variables as pH, accumulation potential, accumulation time and scan rate were studied to attain the optimum conditions of analysis. While for method B, potentiometric determination of DZC was achieved by the use of ion selective electrodes ISEs; the anionic exchanger of ammonium renikate (AR) and phosphotungestic acid (PTA), respectively. Optimization of conditions affecting the potentiometric method has been developed as the studied electrodes and the effect of pH. The electrochemical methods were evaluated according to IUPAC recommendations. These methods were validated by ICH guidelines and capable of being used easily for sensitive determination of DZC in pure form, pharmaceutical formulation and in biological fluids; serum and urine.