Optimization of detection of a novel metabolic syndrome related gene (MSRG) by taqman real-time PCR

Aim To optimize the taqman real-time PCR reaction system for quantitative detection of the expression of MSRG mRNA and evaluate the results.Methods Specific primers and probes were designed and real-time PCR was used for d etection of cDNA sequence.Taqman real-time PCR reaction system from template,primer,probe and Mg~(2+) concentration were optimized.Results Quantity of template was 2μl.The concentratons of primers,probes and Mg~(2+) were 0.4 μmol/L,0.2 μmol/L and 4mM.Conclusion Via optimizing the real-time PCR assay a specific,reliable tool for detecting MRSG mRNA expression levels.was established.