促炎细胞因子
巨噬细胞极化
细胞生物学
巨噬细胞
材料科学
刺激
极化(电化学)
M2巨噬细胞
癌症研究
炎症
化学
生物物理学
免疫学
生物
神经科学
生物化学
体外
物理化学
作者
Ying Kong,Feng Liu,Baojin Ma,Jiazhi Duan,Wenhu Yuan,Yuanhua Sang,Lin Han,Shuhua Wang,Hong Liu
标识
DOI:10.1002/advs.202100962
摘要
Proinflammatory (M1) macrophages play a vital role in antitumor immunity, and regulation of proinflammatory macrophage polarization is critical for immunotherapy. The polarization of macrophages can be regulated by biological or chemical stimulation, but investigations of the regulatory effect of physical stimulation are limited. Herein, regulating macrophage polarization with localized electrical signals derived from a piezoelectric β-phase poly(vinylidene fluoride) (β-PVDF) film in a wireless mode is proposed. Charges released on the surface of the β-PVDF film driven by ultrasonic irradiation can significantly enhance the M1 polarization of macrophages. Mechanistic investigation confirms that electrical potentials rather than reactive oxygen species and mechanical forces enable Ca2+ influx through voltage-gated channels and establishment of the Ca2+-CAMK2A-NF-κB axis to promote the proinflammatory macrophage response during ultrasound treatment. Piezoelectric material-mediated electrical signal-activated proinflammatory macrophages significantly inhibit tumor cell proliferation. A method for electrogenetic regulation of immune cells as well as a powerful tool for engineering macrophages for immunotherapy is provided here.
科研通智能强力驱动
Strongly Powered by AbleSci AI