The proximate, elemental composition and antimicrobial properties of banana peels was investigated using A.O.A.C standard methods. The antimicrobial activity effect of aqueous extract of fresh and dry banana peel against bacteria including (staphylococcus aureus, pseudomonas aeruginosa, and Escherichia coli) was carried out by the well-agar diffusion method. The water extract of dry banana peel showed various inhibitory effects against staphylococcus aureus with a zone diameter of (8mm in 50ug/ml) and pseudomonas aeruginosa with a zone diameter of (10mm in 100ug/ml), with no effect against (Escherichia coli) which was soaked for 24 hours, and the aqueous banana peel extract shows no inhibitory concentration on the fresh banana peel. Aqueous extract of banana can be utilized in cosmetics formulations against both gram-positive and negative bacteria to replace the synthetic chemical formulations in the cosmetics industry.