Metabolic Checkpoint for Cancer Immunotherapy

This repository contains the analysis code used to support the manuscript “In Vivo CRISPR Screens Identify SLC1A5 as a Metabolic Checkpoint for Cancer Immunotherapy.” The code reproduces the main computational analyses, including in vivo CRISPR screen downstream analysis, proteomic pathway enrichment, metabolomic volcano plots, bulk RNA-seq differential-expression analysis, public immunotherapy cohort analysis, TCGA/GTEx expression analysis, single-cell and spatial transcriptomic analyses, and scRNA-seq visualization and cell-composition analyses. Together, these analyses support the identification of tumor cell–intrinsic SLC1A5 as a metabolic regulator of tumor immune evasion and response to immune checkpoint blockade.