First Report of Fusarium brachygibbosum Causing Maize Stalk Rot in China

HomePlant DiseaseVol. 101, No. 5First Report of Fusarium brachygibbosum Causing Maize Stalk Rot in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium brachygibbosum Causing Maize Stalk Rot in ChinaL. Y. Shan, W. Y. Cui, D. D. Zhang, J. Zhang, N. N. Ma, Y. M. Bao, X. F. Dai, and W. GuoL. Y. ShanSearch for more papers by this author, W. Y. CuiSearch for more papers by this author, D. D. ZhangSearch for more papers by this author, J. ZhangSearch for more papers by this author, N. N. MaSearch for more papers by this author, Y. M. BaoSearch for more papers by this author, X. F. DaiSearch for more papers by this author, and W. GuoSearch for more papers by this authorAffiliationsAuthors and Affiliations L. Y. Shan W. Y. Cui D. D. Zhang J. Zhang N. N. Ma Y. M. Bao X. F. Dai W. Guo , Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, P.R. China. Published Online:17 Feb 2017https://doi.org/10.1094/PDIS-10-16-1465-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Stalk rot caused by Fusarium spp. is one of the most destructive diseases in maize growing regions worldwide. This disease may extensively reduce crop yields by interference with absorption and translocation of water and nutrients during grain filling, plant lodging, and premature plant death. Maize plants showing disintegration of the internal stalk tissues were collected from a field in Xinxiang, Henan Province, China, in August 2014. Pith tissues (∼5 mm2) were dissected from the edge of lesions, superficially disinfected with 1% NaClO for 30 s, and 70% ethanol for 30 s. After that, they were rinsed three times in sterile distilled water and dried with filter paper. Three pieces were placed onto Petri dishes containing potato dextrose agar (PDA) amended with streptomycin sulfate (150 µg/ml) and kanamycin (150 µg/ml). The PDA plates were then incubated at 25°C for 4 days. Fungal colonies with morphological characteristics of Fusarium spp. were subcultured by single spore isolation technique. Morphological features of the fungal isolates were observed on PDA and carnation leaf agar (CLA). White colonies with abundant aerial mycelium produced red pigmentation on PDA. Macroconidia produced on CLA were slightly curved, mostly five marked septa, wide central cells, slightly sharp apexes, basal cells with foot-like shape, and measuring 17.6 ± 3.8 µm × 2.7 ± 0.5 µm. Microconidia were rarely observed on either PDA or CLA. Spherical chlamydospores with 6.3 ± 0.6 µm × 6.5 ± 0.5 µm were produced from mycelium in all isolates. These structures were intercalary or terminal, single, and in chains. Morphological characteristics of the isolates were similar to the features of Fusarium brachygibbosum previously described by Padwick (1945). Species identification was confirmed by partial sequences of the translation elongation factor (EF1-α) gene (EF1 and EF2 primers), largest subunit of the RNA polymerase gene (RPB1) (Fa and G2R primers), and second largest subunit of the RNA polymerase gene (RPB2) (5f2 and 7cr primers), respectively (O’Donnell et al. 2010). The isolates showed 99.7, 99.68, and 99.66% identity to those of F. brachygibbosum (FD_01841 for EF-1a, FD_01841 for RPB1, and FD_09786 for RPB2, respectively) in Fusarium-ID database (Geiser et al. 2004). The sequences of partial EF1-α, RBP1, and RBP2 from four different isolates were deposited in GenBank (accession nos. KX984345–56). Pathogenicity tests were performed by inoculating 10-leaf stage maize plants at the second or third internode above the soil line using 20 µl macroconidia suspension at a concentration of 106/ml as described by Zhang et al. (2016). Maize stalks inoculated with sterilized water were used as a control. Each isolate and control was inoculated on three plants. After 10 days, all inoculated stalks showed internal dark brown necrotic regions around the insertion, whereas no symptoms were observed in control plants. The fungus was reisolated from symptomatic tissues but not from the control. It was identified by sequencing partial EF1-α gene again. To our knowledge, this is the first report of maize stalk rot caused by F. brachygibbosum in China.References:Geiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110:473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0 Crossref, ISI, Google ScholarO’Donnell, K., et al. 2010. J. Clin. Microbiol. 48:3708. https://doi.org/10.1128/JCM.00989-10 Crossref, ISI, Google ScholarPadwick, G. W. 1945. Mycol. Pap. 12:11. Google ScholarZhang, Y., et al. 2016. PLoS Pathog 12:e1005485. https://doi.org/10.1371/journal.ppat.1005485 Crossref, ISI, Google ScholarThis research work was supported by the National Basic Research and Development Program of China (973 program, No. 2013CB127803) and Elite Youth Program of Chinese Academy of Agricultural Sciences for Wei Guo.DetailsFiguresLiterature CitedRelated Vol. 101, No. 5 May 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 14 Apr 2017Published: 17 Feb 2017First Look: 27 Dec 2016Accepted: 16 Dec 2016 Page: 837 Information© 2017 The American Phytopathological SocietyCited byThe Potency of Graphitic Carbon Nitride (gC3N4) and Bismuth Sulphide Nanoparticles (Bi2S3) in the Management of Foliar Fungal Pathogens of Maize15 March 2023 | Applied Sciences, Vol. 13, No. 6Identification of Pathogens and Evaluation of Resistance and Genetic Diversity of Maize Inbred Lines to Stalk Rot in Heilongjiang Province, ChinaJinxin Liu, Yujun Han, Wenqi Li, Tiancong Qi, Jiuming Zhang, and Yonggang Li17 February 2023 | Plant Disease, Vol. 107, No. 2Fusarium in maize during harvest and storage: a review of species involved, mycotoxins, and management strategies to reduce contamination23 July 2022 | European Journal of Plant Pathology, Vol. 164, No. 2The distribution and type B trichothecene chemotype of Fusarium species associated with head blight of wheat in South Africa during 2008 and 200926 September 2022 | PLOS ONE, Vol. 17, No. 9Desert soil fungi isolated from Saudi Arabia: cultivable fungal community and biochemical productionSaudi Journal of Biological Sciences, Vol. 29, No. 4Fusarium brachygibbosumCABI Compendium, Vol. CABI CompendiumGenome Sequence Resource of Fusarium brachygibbosum Padwick Strain HN-1, a Causal Agent of Maize Stalk Rot DiseaseHafiz Abdul Haseeb, Sajjad Hyder, Meixu Gao, and Wei Guo20 January 2022 | Plant Disease, Vol. 106, No. 1Amik Ovası Havuç Ekim Alanlarında Sorun Olan Fungal ve Bakteriyel Hastalık Etmenlerin Belirlenmesi13 December 2021 | Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa DergisiFirst Report of Root Rot of Tobacco Caused by Fusarium brachygibbosum in ChinaR. 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