Fluorescence detection of vitamin B12 in human plasma and urine samples using silver nanoparticles embedded in chitosan in micellar media

A novel fluorescence method has been developed for the determination of vitamin B12. The method is based on the interaction of vitamin B12 with silver nanoparticles embedded in chitosan (AgNPs–CH) followed by cloud point extraction using Triton X-100 as the nonionic surfactant.