Identification, saltiness-enhancing effect, and antioxidant properties of novel saltiness-enhancing peptides from peanut protein

. Among them, DPSPR exhibited the most pronounced effect in enhancing saltiness, capable of replacing approximately 56.7% of NaCl. Molecular docking and dynamics simulation studies indicated that amino acid residues Arg272, Glu161, Gln279, Arg168, and Ser165 were found to play key roles in ligand-receptor binding. Additionally, antioxidant activity assays demonstrated that the peptide QPGDY contributed to free radical scavenging in a dose-dependent manner through the hydrogen atom transfer mechanism. The combination of virtual screening technology and experimental validation greatly improved the efficiency and accuracy of peptide discovery and functional characterization, offering a promising strategy for the development of low-sodium foods with antioxidant properties.