Metabolite Profiling and Identification

Understanding the metabolic pathways and metabolite structures is crucial for lead compound design and optimization, safety assessment in drug development, and supporting IND and/or NDA filings. This chapter introduces advanced metabolite profiling and identification (MetID) methods, including the high-throughput identification of metabolic soft spots, the application of metabolite biosynthesis technology, and the detection of reactive metabolites in drug research and development. In addition, the assessment of acyl glucuronide metabolites and deuterated drug strategy from metabolic perspective are also discussed. Collectively, these articles shed light on innovative approaches and technologies that accelerate drug development.