嘧啶二聚体
光防护
环丁烷
嘧啶
DNA
化学
DNA损伤
光解酶
DNA修复
人体皮肤
辐照
光化学
生物物理学
表皮(动物学)
分子生物学
生物化学
生物
遗传学
解剖
有机化学
戒指(化学)
光合作用
物理
核物理学
作者
Thierry Douki,Daniel Bacqueville,Carine Jacques,Camille Géniès,Nicolas Roullet,S. Bessou‐Touya,Hélène Duplan
摘要
Abstract In recent years, interest is growing in the biological cutaneous effects of high‐energy visible light (400–450 nm). In the present study, we explored the impact of blue light (BL) on the repair of pyrimidine dimers, the major class of premutagenic DNA damage induced by exposure to sunlight. We unambiguously demonstrate that the exposure of in vitro reconstructed human epidermis to environmentally relevant doses of BL strongly decreases the rate of repair of cyclobutane pyrimidine dimers and pyrimidine (6‐4) pyrimidone photoproducts induced by a subsequent UVB irradiation. Using the highly sensitive and specific liquid chromatography‐tandem mass spectrometry assay, we did not observe induction of pyrimidine dimers by BL alone. Finally, we showed that application, during the BL exposure step, of a formula containing a new filter, named TriAsorB and affording BL photoprotection, prevented the decrease in DNA repair efficiency. These results emphasize the potential deleterious effects of BL on DNA repair and the interest in providing adequate skin protection against this wavelength range of sunlight.
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