Mercury(编程语言)
胸腺嘧啶
检出限
化学
荧光
选择性
DNA
生物化学
色谱法
计算机科学
物理
催化作用
量子力学
程序设计语言
作者
Ruoying Huang,Mengyan Li,Zhongquan Qu,Yang Liu,Xiaoxing Lü,Ruimin Li,Li Zou
标识
DOI:10.1016/j.foodres.2024.114058
摘要
In this work, we developed a novel label-free fluorescent sensor for the highly sensitive detection of mercury ions (Hg2+) based on the coordination chemistry of thymine-Hg2+-thymine (T-Hg2+-T) structures and the properties of CRISPR-Cas12a systems. Most notably, two T-rich sequences (a blocker and an activator) were designed to form stable double-stranded structures in the presence of Hg2+ via the T-Hg2+-T base pairing. The formation of T-T mismatched double-stranded DNA between the blocker and the activator prevented the cleavage of G-rich sequences by Cas12a, allowing them to fold into G-quadruplex-thioflavin T complexes, resulting in significantly enhanced fluorescence. Under the optimized conditions, the developed sensor showed an excellent response for Hg2+ detection in the linear range of 0.05 to 200 nM with a detection limit of 23 pM. Moreover, this fluorescent sensor exhibited excellent selectivity and was successfully used for the detection of Hg2+ in real samples of Zhujiang river water and tangerine peel, demonstrating its potential in environmental monitoring and food safety applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI