SIRT3
安普克
睡眠剥夺
MFN2型
海马结构
氧化应激
人参
下调和上调
线粒体
内分泌学
医学
药理学
细胞生物学
内科学
神经科学
认知
化学
生物
线粒体融合
基因
蛋白激酶A
生物化学
线粒体DNA
磷酸化
酶
病理
锡尔图因
NAD+激酶
替代医学
作者
Ning Jiang,Caihong Yao,Yiwen Zhang,Xinran Sun,Muhammad Iqbal Choudhary,Xinmin Liu
标识
DOI:10.1021/acs.jafc.3c04618
摘要
Ginsenoside Rg1 (Rg1) is the main bioactive ginseng component. This study investigates the effects of Rg1 on cognitive deficits triggered by chronic sleep deprivation stress (CSDS) and explores its underlying mechanisms. Rg1 effectively improved spatial working and recognition memory, as evidenced by various behavioral tests. RNA-sequence analysis revealed differential gene expression in the metabolic pathway. Treatment with Rg1 abrogated reductions in SOD and CAT activity, lowered MDA content, and increased Nrf2 and HO-1 protein levels. Rg1 administration alleviated hippocampal mitochondrial dysfunction by restoring normal ultrastructure and enhancing ATP activities and Mfn2 expression while regulating Drp-1 expression. Rg1 mitigated neuronal apoptosis by reducing the Bax/Bcl-2 ratio and the levels of cleaved caspase-3. Additionally, Rg1 upregulated AMPK and SIRT3 protein expressions. These findings suggest that Rg1 has potential as a robust intervention for cognitive dysfunction associated with sleep deprivation, acting through the modulation of mitochondrial function, oxidative stress, apoptosis, and the AMPK-SIRT3 axis.
科研通智能强力驱动
Strongly Powered by AbleSci AI