Fe(III) transporter OsYSL15 may play a key role in the uptake of Cr(III) in rice (Oryza sativa L.)

Due to the widely discharge of chromium (Cr) by mining and smelting industries, etc., contamination of paddy soils and rice has become serious problems. Therefore it is crucial to explore how rice takes up Cr. Cr(III) is the most common Cr form in the long-term water flooding paddy soils. Here, we demonstrate that OsYSL15, a key gene for Fe(III) uptake, is equally applicable for Cr(III) uptake in rice. Firstly, the antagonistic effect of Cr(III) and Fe(III) in the uptake process was found. Rice could accumulate more Cr(III) under Fe-deficient conditions. And the Fe(III) content in the protoplasts of rice root cells gradually decreased with the increase exposure of Cr(III). Knockdown of OsYSL15 in rice significantly reduced the Cr(III) uptake rate. Compared with wild type rice, the accumulation of Cr(III) in OsYSL15 mutant was decreased by 40.7%-70.6% after gene editing. These results indicate that OsYSL15 is a key gene responsible for Cr(III) uptake in rice, which can guide the screening or genetic modification for low-Cr-accumulation rice varieties. Long-term consumption of agricultural products produced from Cr-contaminated areas can be hazardous to human health, and rice is the main source of chromium intake. This article first discovered the key gene for Cr(III) uptake in rice, which can provide a theoretical basis for future genetic modification of rice. Therefore, through the findings of this study, it is possible to design crops with low Cr(III) accumulation by manipulating OsYSL15 and its homologous genes. In addition, it can also be overexpressed and transformed into hyperaccumulator plants to remediate Cr(III) pollution in the environment.