The Production of Soluble Human Gamma Interferon in the Escherichia coli Expression System with a Decrease in Cultivation Temperature

A recombinant strain for producing human gamma interferon (IFN-γ) E. coli BL 21/pET-IFN-γ was constructed to provide a high level of its expression. A method has been developed for obtaining a soluble form of recombinant IFN-γ, consisting of the processes of producing a biomass of a producer strain containing a target protein at a level of 32–37% of the total content of cellular proteins, protein isolation, and purification. The purification process included the stages of disintegration, clarification of the cell lysate, chromatographic purification, and dialysis. The developed method makes it possible to obtain up to 5 mg of the drug from 1 g of wet biomass with a purity of at least 95% and high specific (antiviral) activity.