Molecular mechanism of Osthole against chitin synthesis of Ustilaginoidea virens based on combined transcriptome and metabolome analyses

Rice false smut, caused by the fungus Ustilaginoidea virens, is a destructive grain disease in rice-producing areas worldwide. To reveal the action mechanism of osthole against U. virens, the mycelial morphology, differential genes and metabolites of osthole-treated U. virens were determined using electron microscopy and multi-omics, respectively. The hyphae of osthole-treated U. virens were severely wrinkled and distorted with rough cell walls, uneven thickness, and protoplast aggregation. Calcium fluorescent white staining showed that osthole affected chitin synthesis in U. virens. The differential genes and metabolites in U. virens were significantly enriched in amino sugar and nucleotide sugar metabolism pathway. The expression of the acetylglucosamine phosphate mutase (AGM) gene (UvAGM1) and UDP-N-acetylglucosamine was significantly down regulated. The AGM of osthole-treated U. virens was 133.43 ng/mL, which was significantly lower than that of the control group (205.67 ng/mL). Osthole combined with the amino acid residue THR334 of AGM via hydrogen bonding. These results indicate that UvAGM1 may be a key candidate gene of osthole against U. virens. Overall, the results provide valuable information for the application of osthole to control rice false smut.