鼻息肉
基础(医学)
中性粒细胞胞外陷阱
免疫荧光
病理
上皮
生物
发病机制
污渍
增生
免疫学
医学
炎症
抗体
基因
内分泌学
胰岛素
生物化学
作者
Sehan Lim,Roza Khalmuratova,Yun Young Lee,Yi Sook Kim,Mingyu Lee,Na Kyeong Lee,Se−Na Kim,Young Bin Choy,Chun Gwon Park,Dae Woo Kim,Hyun‐Woo Shin
标识
DOI:10.1016/j.jaci.2023.11.016
摘要
Neutrophil extracellular traps (NETs) are observed in chronic rhinosinusitis (CRS), although their role remains unclear.This study aimed to investigate the influence of NETs on the CRS epithelium.Forty-five sinonasal biopsy specimens were immunofluorescence-stained to identify NETs and p63+ basal stem cells. Investigators treated human nasal epithelial cells with NETs and studied them with immunofluorescence staining, Western blotting, and quantitative real-time PCR. NET inhibitors were administered to a murine neutrophilic nasal polyp model.NETs existed in tissues in patients with CRS with nasal polyps, especially in noneosinophilic nasal polyp tissues. p63+ basal cell expression had a positive correlation with the release of NETs. NETs induced the expansion of Ki-67+p63+ cells. We found that ΔNp63, an isoform of p63, was mainly expressed in the nasal epithelium and controlled by NETs. Treatment with deoxyribonuclease (DNase) I or Sivelestat (NET inhibitors) prevented the overexpression of ΔNp63+ epithelial stem cells and reduced polyp formation.These results reveal that NETs are implicated in CRS pathogenesis via basal cell hyperplasia. This study suggests a novel possibility of treating CRS by targeting NETs.
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