Toxicity of microplastic fibers containing azobenzene disperse dyes to human lung epithelial cells cultured at an air-liquid interface

There is growing concern surrounding the human health effects following inhalation exposure to microplastic fibers (MPFs). MPFs can harbor chemical additives, such as azobenzene disperse dyes (ADDs), that may contribute to their toxicity. The goal of this study was to determine the acute biological effects of dyed polyethylene terephthalate MPFs to fully differentiated normal human bronchial epithelial (NHBE) cells cultured at an air-liquid interface. Cells were exposed to 2000 undyed MPFs (84.80 µg/cm