活性氧
抗氧化剂
超氧化物歧化酶
化学
过氧化氢酶
APX公司
NADPH氧化酶
谷胱甘肽
抗坏血酸
谷胱甘肽还原酶
过氧化物酶
谷胱甘肽过氧化物酶
生物化学
食品科学
酶
作者
Xuejiao Zhang,Xiaoyuan Zheng,Ye Han,Ruirui Yang,Qihui Wang,Di Gong,Yongcai Li,Dov Prusky,Yang Bi
标识
DOI:10.1016/j.postharvbio.2023.112308
摘要
Ultraviolet-C (UV-C), a short-wave ultraviolet light, has been extensively proved to regulate reactive oxygen species (ROS) homeostasis and increase antioxidant activity in fruit and vegetables. However, whether UV-C may regulate ROS homeostasis, antioxidant activity and cell membrane integrity at potato tubers wounds during healing has not been reported. In this study, UV-C irradiation activated calcium-dependent protein kinase (CDPK), NADPH oxidase (NOX) and superoxide dismutase (SOD), and promoted superoxide anion (O2.-) and hydrogen peroxide (H2O2) production at tuber wounds. Meanwhile, UV-C activated catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR), and elevated the levels of ascorbic acid (AsA), reduced glutathione (GSH), total phenolic and flavonoid at tuber wounds. Furthermore, UV-C decreased malondialdehyde (MDA) content and cell membrane permeability at tuber wounds by enhancing the scavenging capacities of DPPH, ABTS+ and FRAP. Taken together, UV-C irradiation could maintain cell membrane integrity at potato tubers wounds during healing by regulating ROS homeostasis and increasing antioxidant activity.
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