In this paper, we introduce some monotonicity rules for the ratio of integrals. Furthermore, we demonstrate that the function -Tν,α,β(s) is completely monotonic in s and absolutely monotonic in ν if and only if β≥1 , where Tν,α,β(s)=Kν2(s)-βKν-α(s)Kν+α(s) defined on s>0 and Kν(s) is the modified Bessel function of the second kind of order ν . Finally, we determine the necessary and sufficient conditions for the functions s↦Tμ,α,1(s)/Tν,α,1(s) , s↦(Tμ,α,1(s)+Tν,α,1(s))/(2T(μ+ν)/2,α,1(s)