共焦
共焦显微镜
显微镜
荧光寿命成像显微镜
转录组
生物医学工程
原位
人工智能
计算机科学
材料科学
计算机视觉
计算生物学
荧光
生物
细胞生物学
光学
化学
医学
物理
基因表达
基因
生物化学
有机化学
作者
Rongxin Fang,Aaron R. Halpern,Mohammed Mostafizur Rahman,Zhengkai Huang,Zhiyun Lei,Sebastian J. Hell,Catherine Dulac,Xiaowei Zhuang
标识
DOI:10.1101/2023.07.21.550124
摘要
Abstract Multiplexed error-robust fluorescence in-situ hybridization (MERFISH) allows genome-scale imaging of RNAs in individual cells in intact tissues. To date, MERFISH has been applied to image thin tissue samples of ∼10-µm thickness. Here, we present a method to enable three-dimensional (3D) single-cell transcriptome imaging of thick tissue specimens by integrating MERFISH with confocal microscopy for optical sectioning and deep learning for increasing imaging speed and quality. We demonstrated 3D MERFISH on mouse brain tissue sections of up to 200 µm thickness with high detection efficiency and accuracy. We anticipate that 3D thick-tissue MERFISH imaging will broaden the scope of questions that can be addressed by spatial genomics.
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